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Online Hizmetlere Toplu BakışTurkish Journal of Hematology
Turk J Hematol. 2008; 25(4): 172-175 | |||
Mezankimal kök hücrelerin in vivo takibinde yeşil floresan protein aktarılmasının optimizasyonuYusuf Baran1, Ali Uğur Ural2, Ferit Avcu2, Meral Sarper3, Pınar Elçi3, Aysel Pekel41Medical And Cancer Research Center, Gülhane Military Medical School, Ankara, Turkey. Molecular Biology And Genetics Department, İzmir Institute Of Technology, İzmir, Turkey2Medical And Cancer Research Center, Gülhane Military Medical School, Ankara, Turkey. Hematology Department Gülhane Military Medical School, Ankara, Turkey 3Medical And Cancer Research Center, Gülhane Military Medical School, Ankara, Turkey 4Immunology Department Gülhane Military Medical School, Ankara, Turkey AMAÇ: Yeşil floresan proteini (YFP), günümüzde hücre biyolojisi çalışmalarında tanımlayıcı gen ve hücre işaretleyici olarak kullanılmaktadır. YFP’nin oldukça önemli kullanım alanları farklı genlerin içerisine eklenerek bu genlerin farklı organizmalardaki ekspresyonlarının miktar tayininde ve canlı hücreler içerisinde işaretleyici olarak kullanılabilmesidir. Bu çalışmamızda doku tamiri amacıyla ve hayvanlara aktardığımız mezankimal kök hücrelerini (MKH) in vivo takip edebilmek amacı ile YFP genini içeren plazmid vektörünün MKH’lara aktarılmasını optimize etmeye çalıştık. Optimization of transfection of green fluorescent protein in pursuing mesenchymal stem cells in vivoYusuf Baran1, Ali Uğur Ural2, Ferit Avcu2, Meral Sarper3, Pınar Elçi3, Aysel Pekel41Medical And Cancer Research Center, Gülhane Military Medical School, Ankara, Turkey. Molecular Biology And Genetics Department, İzmir Institute Of Technology, İzmir, Turkey2Medical And Cancer Research Center, Gülhane Military Medical School, Ankara, Turkey. Hematology Department Gülhane Military Medical School, Ankara, Turkey 3Medical And Cancer Research Center, Gülhane Military Medical School, Ankara, Turkey 4Immunology Department Gülhane Military Medical School, Ankara, Turkey OBJECTIVE: Green Fluorescent Protein (GFP) has been used as a marker of gene expression and a single cell marker in living organisms in cell biology studies. The important areas that GFP is used are expression levels of different genes in different organisms by inserting GFP in these genes and as a marker in living cells. In this study, we tried to optimize transfection of mesenchymal stem cells, (MSCs) used for regeneration of damaged tissues in animals, by GFP containing plasmid vector by which MSCs can be followed in vivo. Yusuf Baran, Ali Uğur Ural, Ferit Avcu, Meral Sarper, Pınar Elçi, Aysel Pekel. Optimization of transfection of green fluorescent protein in pursuing mesenchymal stem cells in vivo. Turk J Hematol. 2008; 25(4): 172-175 Sorumlu Yazar: Ali Uğur Ural, Türkiye |
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