Serum Prolidase Activity And Oxidative Status In Lung Cancer Cell Line (A549)Hasan Dağli1, Metin Kilinc1, Ebru Temiz2, Kaifee Arman2, Uğur Arslanyürekli2
2Department of Medical Biology, University of Gaziantep, Gaziantep, Turkey
Non-small cell lung cancer is a disease in which malignant (cancer) cells develop in the tissues of the lung. There are a few types of non-small cell lung cancer. Smoking is the major risk factor for non-small cell lung cancer. Prolidase is a cytosolic imidodipeptidase, which specifically splits imidodipeptides with C-terminal proline or hydroxyproline 3. The enzyme prolidase plays an critical role in the breakdown of collagen as well as the intracellular protein especially in the last stage when peptides and dipeptides implicate a high level of proline. The aim of this study was to determine the levels of malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and the relationship of antioxidant enzyme levels with serum prolidase levels in lung cancer cell line. For this purpose, the lung cancer cell line (A549) and epithelial bronchial cell line (BEAS-2B) were exposed to miRNA targeting MALAT1 in the cell cultur medium. Serum prolidase activity was measured spectrophotometrically. The determinations of MDA and antioxidant enzymes were performed spectrophotometrically. The results showed a significant decrease in SOD and CAT activities in A549 compared to the BEAS-2B. The present study demonstrate that serum prolidase activity and oxidative stress are significantly associated with the lung cancer and that the correlation between serum prolidase activity decreased serum level and markers of oxidative stress are represented as increased.