[doi: 10.5505/2017ichc.PP-38]

Prominent Roles of miR-503 and miR-15a in Breast Cancer (MCF-7) affecting Cell Cycle but not Apoptosis

Hasan Dağlı1, Seher Kara Öngel1, Kaifee Arman2
1Department Of Medical Biochemistry, University Of Kahramanmaraş Sütçü Imam University, Kahramanmaraş, Turkey
2Department Of Medical Biology, University Of Gaziantep, Gaziantep, Turkey

Breast cancer is dreadful cancer among women worldwide. miRNAs play an important role in breast cancer. For this purpose, the breast cancer cell line (MCF-7) and epithelial breast cell line (CRL-4010) were exposed to three different mimic miRNAs i.e. miR-503, miR-150 and miR-15a in the cell culture medium. Flow cytometry was used to determine cell cycle and the level of apoptosis in both cell lines. It was found that cells had no considerable effect on apoptosis using these miRNAs. There was almost no change in cells undergoing apoptosis in both cell lines after using these miRNAs. After analysing cell cycle, it was noticed that miR-503 affects the cell cycle of MCF-7 maximum by inhibiting the progression of S phase and G2/M phase as compared to CRL-4010. G0/G1 phase was inhibited maximum for miR-15a. It was concluded that the used three different miRNAs inhibited the progression of cell cycle at different phases i.e miR-503 at S and G2/M phase and miR-15a at G0/G1 phase as compared to CRL-4010. It was found that miR-150 has no effect on cell cycle in MCF-7 and CRL-4010.