[doi: 10.5505/2017ichc.PP-269]
Immunohistochemical study of Osteopontin and Bcl-2 gene expression in kidney tissue in histidine-tryptophan-ketoglutarate (HTK) solution prepared with N-acetyl-L-carnitine
Candan Özoğul1, Ilkay Başer Demircan1, Süreyya Barun2, Mustafa Kavutçu3, Mustafa Ilhan4, Seda Nur Akyol1, Ibrahim Murat Hirfanoğlu5, Mustafa Bilgi3, Gülistan Sanem Sarıbaş1, Pınar Kaçamak1, Asiye Aslı Emniyet12Gazi University, Faculty of Medicine, Division of Basic Medical Sciences, Department of Medical Pharmacology, Ankara, Turkey
3Gazi University, Faculty of Medicine, Division of Basic Medical Sciences, Department of Medical Biocemistry, Ankara, Turkey
4Gazi University, Faculty of Medicine, Division of Basic Medical Sciences, Department of Public Health, Ankara, Turkey
5Gazi University, Faculty of Medicine, Division of Internal Medical Sciences, Department of Pediatric Health and Illnesses, Ankara, Turkey
In this study we aimed to determine Osteopontin and Bcl-2 expression levels in kidney tissue in histidine-tryptophan-ketoglutarate (HTK) solution prepared with Acetyl L-Carnitine as immunohistochemistrical.
Organ transplantion is main solution for organ failure. Transplant organ must be protected from hypoxia to avoid ischemia.Ischemia is a restriction in blood flows to tissues, trigger to produce of reactive oxygen species (ROS), proinflammatory cytokine production and caused activation of apoptotic pathway. To prevent these damages, preservation solution is used include HTK that a protective agent, plays an important role in mitochondrial functions. Osteopontin (OPN) is an extracellular matrix cell adhesion phosphoglycoprotein. It is generally produced by osteoblasts and also produced by brain and kidney. OPN has some renoprotective actions in renal injury, such as increasing tolerance to acute ischemia, decreasing cell apoptosis and participating in the regeneration of cells. Bcl-2 is an antiapoptotic proteins are responsible to supress otophagy and apoptosis and allow the cell to survive.
MATERIAL-METHODS: We used 24 female rats equally divided into four groups: group1 had the uterus stored in HTK solution at +4°C cold storage for 4hours. Group2, the uterine tissue was stored in HTK solution combined with Acetyl L-carnitine for 4hours at +4°C. The same procedure with group1/2 were repeated for 24hours for groups3/4 respectively. Histological investigation and immunohistochemical analysis were performed.
RESULTS: Glomerular sclerosis was found to be mild in short-term HTK and HTK+L-Car groups but it was found widely in long term HTK and HTK+L-Car groups on histologic evaluation and widely in long term HTL+L-Car. Tubuler cell vacuolization and cellular desquamation were observed in the proximal tubules in the long-term HTK group. According to immunohistochemical evaluation, in short and long term HTK+L-Car groups acetyl L-Carnitine prevented the antiapoptotic mechanisms to be activated and the intense expression of Bcl-2 has not occurred. In short- and long-term HTK groups, Osteopontin showed more immunopositive result, as a marker of tissue damage.
CONCLUSIONS: It was determined that the modified HTK solution prevented the increase of the activation of the expected oxidant mechanisms resulting in ischemia. This contribution of acetyl L-carnitine was also found in long-term group findings.
Histological and immunohistochemical examination of all the groups.
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