[doi: 10.5505/2017ichc.PP-143]Expression pattern of miR-23b-3p and its target SETD2 in Renal Cell CarcinomaRozhgar A. Khailany1, Naser Gilani1, Belan O. Kanabe2, Khandakar A. S. M. Saadat1, Ahmet Arslan32Department of Biology, Gaziantep University, Gaziantep, Turkey 3Department of Medical Biology, Faculty of Medicine, Namık Kemal University, Tekirdag, Turkey Renal cell carcinoma (RCC) is the most common type of kidney cancer in adults. It composes around 3% of adult malignancies and 90-95% neoplasms of kidney. MicroRNA-23b-3p, a small non-coding RNA, is up-regulated in different cancers. Also it is predicted that tumor suppressor gene SET domain-containing protein 2 (SETD2) is a direct target of this miRNA. SETD2 encodes a histone methyltransferase, which is responsible for trimethylation of the lysine residue at position 36 of histone H3 and may play a role in suppressing tumor development. The aim of this work was to measure the expression level of miR-23b-3p and its target SETD2 in patients with renal cell carcinoma and normal control sample. Twenty one paired tumor and normal tissue samples that were grouped according to the sub-types of renal carcinoma and clinical characteristics of patients, including gender and average age were observed with gene expression analysis using quantitative real time polymerase chain reaction (qRT-PCR). The expression of SETD2 is quite lower as compared to miR-23b-3p expression in RCC. As a result, we conclude that miR-23b-3p may have an important role in suppressing the expression of SETD2. The aberrant expression in miR-23b-3p and its target SETD2 might be a risk factor for RCC. To our knowledge, it is the only study in which the expression levels of SETD2 and miR-23b-3p in RCC at the same time have been evaluated. Further analysis is required to determine the responsible miRNAs and their targets rather than miR-23b-3p and SETD2 in RCC. |